LOCUS       V003758                 6140 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003758
VERSION     V003758
KEYWORDS    beta-galactosidase; beta-lactamase; rRNA methylase; rep protein
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Ishikawa J, Niino Y, Hotta K.
  TITLE     Construction of pRES18 and pRES19, Streptomyces-Escherichia coli
            shuttle vectors carrying multiple cloning sites
  JOURNAL   FEMS Microbiol. Lett. 145 (1), 113-116 (1996)
   PUBMED   8931335
REFERENCE   2  (bases 1 to 6140)
  AUTHORS   Ishikawa J, Sudoh M, Hotta K.
  TITLE     Nucleotide sequence of the pRES19
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6140)
  AUTHORS   Ishikawa J, Sudoh M.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2000) Jun Ishikawa, National Institute of
            Infectious Diseases, Department of Bioactive Molecules; 1-23-1
            Toyama, Shinjuku, Tokyo 162-8640, Japan (E-mail:jun@nih.go.jp,
            URL:http://www.nih.go.jp/niid/index-e.html,
            Tel:81-3-5285-1111(ex.2354), Fax:81-3-5285-1272)
REFERENCE   4  (bases 1 to 6140)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6140)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS
            Microbiol. Lett."; date: "1996"; volume: "145"; issue: "1"; pages:
            "113-116"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (22-MAR-2000) Jun Ishikawa, National Institute of Infectious
            Diseases, Department of Bioactive Molecules"; volume: " 1-23-1
            Toyama, Shinjuku, Tokyo 162-8640, Japan (E-mail:jun@nih.go.jp,
            URL:http://www.nih.go.jp/niid/index-e.html,
            Tel:81-3-5285-1111(ex.2354), Fax"; pages: "81-3-5285-1272"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      425..434
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(1219..2025)
                     /gene="tsnR"
                     /label="23S rRNA (adenosine(1067)-2'-O)-methyltransferase"
                     /note="23S rRNA (adenosine(1067)-2'-O)-methyltransferase
                     from Streptomyces azureus. Accession#: P18644"
     promoter        2703..2807
                     /label="AmpR promoter"
     CDS             2808..3665
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3839..4427
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    4715..4736
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4751..4781
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4789..4805
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4813..4829
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(4842..4898)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(4899..4915)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 tgatcagcga ggcgacaccc acagcgatct cgttcgccct cttggcgcgg atcttggccg
       61 agcagaccgg gcagagccag ccgttcgcac agatgcagag cccggacgtg taggccaccc
      121 cgtcacgtac gtggatcttg acccggtcca ccttgccgtt ctggccctga gccatgaccc
      181 gtccgcaggc cccgagacgc ttgaccgtcg tgaagctgac gatggcccgc tgggcgtcgt
      241 accggcgacg acgtcggcaa cacggatcgg tgcccttccc gcattcgtgc tggtcagacc
      301 cggtttcccc cgaggggata gaaacaaagt ccgtagtagt accaagcggg gcgtccgcca
      361 ggccgccaga ggcggcccgg cgcccctcaa gggcttcgcc aacggggcgc cgggagacct
      421 ggcggccacc atgggcccgc aggtgtttcg cggcctgtgc cccatccagc gggaggaccg
      481 ggggcacgtc atcatgcggg gtggctacct gtggtgccgg acccttgccg tgctctaccg
      541 tggtcatcga tcaacttctc cgttcgcgtt ccaggtggcg gaggaagagg cccggaccgt
      601 cgtcagcggt tccgggcctc cgtcgttcca tgcgatcctg acggccgtgg ccgaccggcg
      661 agctatggac tcgccgggcg gtcacttctc cgtctgcgag cccatcagcg tcttgatgac
      721 cagccagccg atcgcgcagg agatcgcgcc gatgccgacc gcgacggcga cgacggcgac
      781 ggccaggagg acggcgacga ccaggacccc ggcaccgcca gcggccacga tctgtccggc
      841 gtgctgacgg gtccaggagg gcgcaggggc cgcctgctgg acgatgacgt agccgggggt
      901 ctgctggacg ctctgaggga cgctcagcgc ctgtccgggg ccgatgtgaa tgggtgtcgg
      961 ggtgtagacc gtttcggggc cgtaggacgt cgtcacgggg tgcctccgag gtcttccagc
     1021 tcgatggacc agaaccgcgt cagcgggacg acgcgcgaga agttccggcc cgtctccttc
     1081 tccatgcttt cgacggcgag ttccacagcg cggcggtaga tgcgctggcg ggacgttccg
     1141 gccgggacgt cgttagcagt gatcatcact gacgaatcga ggtcgaggaa ccgagcgtcc
     1201 gaggaacaga ggcgcttatc ggttggccgc gagattcctg tcgatcctct cgtgcagcgc
     1261 gattccgagg gaaacggaaa cgttgagaga ctcggtctgg ctcatcatgg ggatggaaac
     1321 cgaggcggaa gacgcctcct cgaacaggtc ggaaggccca cccttttcgc tgccgaacag
     1381 caaggccagc cgatccggat tgtccccgag ttccttcacg gaaatgtcgc catccgcctt
     1441 gagcgtcatc agctgcatac cgctgtcccg aatgaaggcg atggcctcct cgcgaccgga
     1501 gagaacgacg ggaagggaga agacgtaacc tcggctggcc ctttggagac gccggtccgc
     1561 gatgctggtg atgtcactgt cgaccaggat gatccccgac gctccgagcg cgagcgacgt
     1621 gcgtactatc gcgccgatgt tcccgacgat cttcaccccg tcgagaacga cgacgtcccc
     1681 acgccggctc gcgatatcgc cgaacctggc cgggcgaggg acgcgggcga tgccgaatgt
     1741 cttggccttc cgctccccct tgaacaactg gttgacgatc gaggagtcga tgaggcggac
     1801 cggtatgttc tgccgcccgc acagatccag caactcagat ggaaaaggac tgctgtcgct
     1861 gccgtagacc tcgatgaact ccaccccggc cgcgatgctg tgcatgaggg gctcgacgtc
     1921 ctcgatcaac gttgtcttta tgttggatcg cgacggcttg gtgacatcga tgatccgctg
     1981 caccgcggga tcggacggat ttgcgatggt gtccaactca gtcatggtcg tcctaccggc
     2041 tgctgtgttc agtgacgcga ttcctggggt gtgacaccct acgcgacgat ggcggatggc
     2101 tgccctgacc ggcaatcacc aacgcaaggg gaagtcgtcg ctctctggca aagctccccg
     2161 ctcttccccg tccgggaccc gcgcggtcga tccccgcata tgaagtattc gccttgatca
     2221 gggctctcgc ggacatcgga gaggtagccc agcaagcaga gacagcactg tgcgtgaacg
     2281 cggtgaagac gaagtccttg aacccgaccg gcgaagatca cgcggaatgc cgtttacaag
     2341 aggcttgccg ccgaggacaa atgacgacgc gcctgatgcg gtattttctc cttacgcatc
     2401 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
     2461 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     2521 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     2581 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     2641 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     2701 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     2761 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     2821 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     2881 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     2941 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     3001 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     3061 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     3121 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     3181 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     3241 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     3301 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     3361 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     3421 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     3481 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     3541 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     3601 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     3661 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     3721 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     3781 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3841 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3901 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3961 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     4021 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     4081 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     4141 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     4201 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     4261 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4321 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     4381 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4441 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     4501 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     4561 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     4621 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     4681 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     4741 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     4801 aacaatttca cacaggaaac agctatgacc atgattacgc caagcttgca tgcctgcagg
     4861 tcgactctag aggatccccg ggtaccgagc tcgaattcac tggccgtcgt tttacaacgt
     4921 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     4981 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     5041 ctgaatggcg aatggcgcgt cgaaggcccc ctgccagtgt cgggcggggg gccttttctg
     5101 tgattccggg ccgaagaagt ctttacctaa agctgatcag cggggacccc tccgagagca
     5161 agaaaggggg ccgagtgacc actaagggcg gtgagggatt ccatccctct cctttggtac
     5221 aagtgtaaag gccccctttc atgctctcgc cccgctgatc acctttaggt aaacacttct
     5281 ccaaccctaa gcggcattgc tttctgacgg gccgtcaggg gtttctgacg catcatcagg
     5341 tgtgagtttc ttgactgccc agatttgatg agccgtgagg agcttgacga gcgcgggaaa
     5401 gcctccgcgt tcgacggctt ccaggacgcg ggcttccagg ccgtgacgga tgaccatctt
     5461 ccaagcggcc tccgccagct cggccacctc cccgccggcc acttcttcgg cggccaactc
     5521 ttcctcggtc ttctcgtcca ctccgagcat ggctttcagc ttgggagacc accgcaacca
     5581 ggacttcccc ttggtcgcct tctcgtactc cagccagaga tccacgacgg ccgcgtctcc
     5641 ggtcgccatc aggtactcga tcatctcgaa cggcgtcagg ctgccgagct tgcctttctt
     5701 gagatcaccg cgagcaactt cgttcccgat gtgcttgccg gaatcctgag ccttagcgat
     5761 gtattccccg gcaccttcgg cggtcatgac cttgtcccac ttcacaccgt gagccttgtc
     5821 ggcagggaag ccgtgcttct tcgtccaggc catccaaagc cgttgccagc gacccatcat
     5881 gatggcgagt tcccgaacgt cggggacttc cttgaagcag acgatcacgt gaaggtgagg
     5941 atgccaacca ttctttccgt aagtgatctc acgggaattg atggtgccga tctttcctgt
     6001 tgccttcttt tcccggacgg ccataactcc ggtaccgagc tttcggaatg cgtcgttgag
     6061 agcattaagc agatccgcca gagcgtgacc cctcgcgtgc cgaacggtga acgtgaccat
     6121 ccaggcttcc ccaccaccca
//
